polysome lysis buffer (Thermo Fisher)
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polysome lysis buffer
Polysome Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgcl2/MGCL2/pm42844465-903-13-38
Average 99 stars, based on 1 article reviews
Polysome Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mgcl2/MGCL2/pm42844465-903-13-38
Average 99 stars, based on 1 article reviews
polysome lysis buffer - by Bioz Stars,
2026-11
99/100 stars
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Lysis:Article Title: LncRNA CACF promotes autophagy and cardiac recovery after myocardial infarction by targeting ATG7 Article Snippet: .. Next, 806 lysis buffer which containing 20 mM HEPES (Thermo Scientific, USA), 1.5 mM Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation. Article Snippet: .. In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM Article Title: Germline sequence variation within the ribosomal DNA is associated with human complex traits. Article Snippet: Cells were pelleted by centrifugation at 500g for 3 min at 4◦C, and washed twice with ice-cold PBS (Thermo Fisher Scientific Cat. No. 10010023) supplemented with 100μg/ml cycloheximide. .. Lysis buffer consisted of 25 mM Tris-HCl (Thermo Fisher Scientific Cat. No. J60202-K2), 5 mM Incubation:Article Title: LncRNA CACF promotes autophagy and cardiac recovery after myocardial infarction by targeting ATG7 Article Snippet: .. Next, 806 lysis buffer which containing 20 mM HEPES (Thermo Scientific, USA), 1.5 mM Article Title: Rodent-specific induction of cholestasis and cholangioma through the reduction of bile salt export pump by bardoxolone methyl. Article Snippet: .. Thirty minutes after incubation at 37°C under 5% CO2, cells were washed with Dulbecco’s phosphate-buffered saline containing CaCl2 and Membrane:Article Title: LncRNA CACF promotes autophagy and cardiac recovery after myocardial infarction by targeting ATG7 Article Snippet: .. Next, 806 lysis buffer which containing 20 mM HEPES (Thermo Scientific, USA), 1.5 mM Stable Transfection:Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation. Article Snippet: .. In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM Expressing:Article Title: ZAP targets aberrant mRNA transcripts encoding proteins with defective signal peptides for degradation. Article Snippet: .. In total, 50 million K562 ZAP-S KO cells stably expressing HAtagged ZAP-S were harvested and lysed in lysis buffer (50 mM HEPES [pH 8], 150 mM NaCl, 5 mM Extraction:Article Title: Mevalonate kinase represses anthocyanin biosynthesis via sucrose transporters and gibberellin synthesis pathways in arabidopsis. Article Snippet: .. Then, the seedlings were treated with 10 μM GA for 2 h. Total protein was extracted using extraction buffer containing 50 mM Tris–HCl (pH 7.5), 250 mM NaCl, 10 mM Protease Inhibitor:Article Title: Mevalonate kinase represses anthocyanin biosynthesis via sucrose transporters and gibberellin synthesis pathways in arabidopsis. Article Snippet: .. Then, the seedlings were treated with 10 μM GA for 2 h. Total protein was extracted using extraction buffer containing 50 mM Tris–HCl (pH 7.5), 250 mM NaCl, 10 mM Article Title: DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity. Article Snippet: .. Cells were lysed in 5 ml/gram cell pellet in 25 mM HEPES pH 7.9, 150 mM NaCl, 0.5% Triton-X-100, 1 mM EDTA, 1 mM Article Title: Germline sequence variation within the ribosomal DNA is associated with human complex traits. Article Snippet: Cells were pelleted by centrifugation at 500g for 3 min at 4◦C, and washed twice with ice-cold PBS (Thermo Fisher Scientific Cat. No. 10010023) supplemented with 100μg/ml cycloheximide. .. Lysis buffer consisted of 25 mM Tris-HCl (Thermo Fisher Scientific Cat. No. J60202-K2), 5 mM Sonication:Article Title: DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity. Article Snippet: .. Cells were lysed in 5 ml/gram cell pellet in 25 mM HEPES pH 7.9, 150 mM NaCl, 0.5% Triton-X-100, 1 mM EDTA, 1 mM Saline:Article Title: Rodent-specific induction of cholestasis and cholangioma through the reduction of bile salt export pump by bardoxolone methyl. Article Snippet: .. Thirty minutes after incubation at 37°C under 5% CO2, cells were washed with Dulbecco’s phosphate-buffered saline containing CaCl2 and Article Title: Dysregulation of the DNA damage response by phosphorothioate antisense oligonucleotides. Article Snippet: Purified oligonucleotides were freeze-dried, redissolved in 1.5 mL of Milli-Q water, and desalted using NAP columns (NAP-10, Cytiva) according to the standard desalting protocol. .. Cells were grown on sterilized coverslips and thereafter either fixed directly with 4% paraformaldehyde (PFA) for 15 min, followed by permeabilization with 0.1% Triton X-100 in phosphate-buffered saline (PBS) for 5 min, or alternatively, if soluble proteins were to be removed, cells were pretreated with cytoskeleton buffer (CSK; 10 mM pipes (pH 7.0; Sigma-Aldrich), 100 mM NaCl, 300 mM sucrose, 3 mM Fluorescence:Article Title: Rodent-specific induction of cholestasis and cholangioma through the reduction of bile salt export pump by bardoxolone methyl. Article Snippet: .. Thirty minutes after incubation at 37°C under 5% CO2, cells were washed with Dulbecco’s phosphate-buffered saline containing CaCl2 and Microscopy:Article Title: Rodent-specific induction of cholestasis and cholangioma through the reduction of bile salt export pump by bardoxolone methyl. Article Snippet: .. Thirty minutes after incubation at 37°C under 5% CO2, cells were washed with Dulbecco’s phosphate-buffered saline containing CaCl2 and other:Article Title: P Can PIE: A group I intron platform for efficient circRNA synthesis at ambient temperatures Article Snippet: Plasmid templates were linearized by BamHI or EcoRI (New England Biolabs). |